During the reconstruction and expansion of the expressway, the upstream transition zone was set up to ensure the smooth transition of vehicles from the closed lane to the open lane. In this paper, traffic flow operation data of 7 construction schemes of Binlai expressway were collected on the spot, including road widening, bridge widening, interchange reconstruction, lane passage by using the road of opposite side，roadbed elevation, flyover and road to bridge. Single factor analysis of variance showed that there was no significant difference in headway and running speed between interchange reconstruction area and lane passage guide zone. Through the UC-win/Road driving simulation experiment, it is verified that the theoretical calculation results of the length of the upstream transition zone of the three construction schemes of road widening, bridge width and interchange reconstruction meet the requirements of the simulation experiment. In the actual setting, the length of the upstream transition zone of the leading road can be calculated according to the acceptable gap theory.