The issue of excessive vibration in high-rise buildings located in the test track area of subway depots is studying. Through on-site measurement and finite element simulation of the environmental vibration before and after the vibration reduction transformation of a subway vehicle testing line, the vibration source and the vibration transmission law of the upper cover before and after the vibration reduction transformation were analyzed. The results indicated that the influence of vehicle speed on the dominant frequency of the source intensity affects the level of vibration transmitted to various floor slabs. The vibration measured in bedrooms was relatively large under the condition of 60 km/h, while the vibration in living rooms was more significant under the condition of 40 km/h. The vibration decreased first, then increased and then decreased with the increased of the distance between the measuring point and the vibration source. While the steel spring floating slab reduced vibration, noise may be slightly amplified, with all vibration measurement poited at the vibration source reduced by more than 10.00 dB after the modification, but the noise at the 7.5 m measurement point increased by 0.96 dB. By compared and observed the changes in vibration levels at different frequencies with floor height before and after the vibration reduction modification, it was found that the maximum frequency band of vibration acceleration level shifts forward, from 25~40 Hz before the modification to 20~25 Hz after, which was related to the change in excitation frequency at the vibration source. Compared to conventional ballast beds, the steel spring floating slab after vibration reduction modification can significantly reduce vibrations, enabling the buildings constructed above the test track area of this depot to meet the corresponding regulatory requirements.