To obtain better structural performance by exploiting the advantages of different materials, a topologi- cal design method of thermo-mechanical coupling structure using multiple materials under non-uniform temper- ature field is proposed in this study. The objective function was developed by minimizing the strain energy to satisfy the stiffness requirements, and the structure volume was restricted. The multi-material topology optimiza- tion model of thermo-mechanical coupling structure subjected to non-uniform temperature field was also estab- lished. The sensitivities of the objective function and the constraint were solved by the adjoint matrix approach. The Heaviside filter technique was adopted to modify the sensitivities in to avoid the phenomenon of numerical instability and the method of moving asymptotes was used to solve the topological optimization problem. The nu- merical examples were given to illustrate that the proposed method is correct and effective.