Aiming at the problems of low efficiency, long cycle time and high cost for traditional single car brake experiment training, this paper designed and developed a single car brake training system of virtual and reality combination based on Unity3D engine. Taking C64 single car as the object, by using Maya software to build a model and adopting NGUI, DoTween animation, C # and other plug-ins and scripting languages, it created a three-dimensional virtual interactive environment. Meanwhile, the single-chip technology and the serial commu- nication technology were adopted to establish the single car brake experimental training system of virtual and re- ality combination with the simulation of a single car tester and brake experiment as the core. The research re- sults show that compared with the traditional single car brake experiment training method, this system has good operation convenience and learning efficiency, which can meet the needs of users for single car brake experiment training.