Based on evolutionary game theory, with the introduction of reward and punishment mechanisms, an evolutionary game model between online freight platforms and government regulators is constructed, and the stability of their equilibrium points is analyzed. The results show that under static punishment, there is no equilibrium stability point in the system, no matter whether the reward mechanism is static or dynamic; under the static reward dynamic punishment and dynamic reward and punishment mechanism, both sides tend to reach a stable equilibrium point, and the effect is better under the dynamic reward and punishment mechanism; the online freight platforms' behavior is affected by the government′s reward and punishment mechanism, and the binding effect of the punishment mechanism is more significant.