This paper, through analyzing the dissipation of straight non-motor vehicle，in the signalized intersec- tion and the process of passing through non-motor vehicle flow of vehicles turning right, explored the principle of conflict between vehicles and bicycles and put forward a method about delay calculation of the vehicles turn- ing right passing through straight non-motor vehicle flow. Through the Nanjing Xikang Road and West Hankou Road intersection data validation, the research results show that the actual delay of vehicles turning right in the intersection due to the non-motor vehicles and the ratio to the calculated theoretical value was kept at 1±0.05 with the calculation results satisfying the actual value of the delay of vehicles turning right and in the reasonable error range. This method can be used to analyze the intersection delay before and after setting the right turn phase, so as to help to evaluate the overall benefit of the right turn phase.