The ANSYS and the ETABS were adopted to establish finite element model for the Ganjiang bridge in Fushan in this paper. The program compiled by MATLAB was applied to solve the dynamic equation of vehicle bridge coupling. The dynamic response and dynamic impact coefficient of large span continuous box girder bridge with corrugated steel webs under the action of vehicle speed and pavement serviceability were analyzed, and the obtained results were compared with the calculated value in accordance with the current codes. The re- search results show that the effect of vehicle speed on the impact coefficient of large span continuous box girder bridge with corrugated steel webs is complex, and the impact coefficient does not increase linearly with the in- crease of velocity. Besides, the worse the pavement condition is, the more obvious the influence of pavement ser- viceability on impact coefficient of bridge is. For the multi-span continuous box girder bridge with corrugated steel webs, as the vehicle speed and pavement serviceability remain unchanged, the impact coefficients of differ- ent main spans are different. It is suggested that the maximum impact coefficient of main span should be used to design this type of bridge.