In this paper, MoS₂ nanosheets were successfully prepared using liquid phase ice-bath ultra-sonication method. The morphologies, microstructures and thickness of MoS₂ nanosheets were characterized by using SEM, TEM and AFM. The SEM and TEM analysis exhibited that MoS₂ powders were successfully exfoliated into MoS₂ nanosheets. AFM analysis showed that the thickness of MoS₂ nanosheets was the monolayer nanosheets. The tribological results showed that MoS₂ nanosheets were conducive to reducing the friction coefficient under high load and rotational speed, and the friction coefficient was about 0.09. The morphology and composition of the worn surface was examined and analyzed using SEM, and MoS₂ nanosheets could effectively reduce the wear for tribosystem. The investigation could provide the guidance for preparing the MoS₂ nanosheets with superior tribological performance.