The electric vibration exciter was used to perform simple harmonic excitation test on the reinforced truss concrete floor to determine the vibration response of the floor under different amplitude and frequency simple harmonic excitation. It used ANSYS to model and analyze the test piece, compared with the test results to verify the feasibility of the finite element method for dynamic analysis of reinforced concrete slabs. Then, a dynamic analysis model of reinforced truss concrete two-way slab under artificial excitation was established, and the influence of bottom steel plate and slab thickness (truss height) on the vibration performance and comfort of the floor was analyzed, and it was combined with the open profiled steel plate-concrete of the same specification. Comparative analysis of floor slabs shows that under the same conditions, the fundamental frequency of reinforced truss concrete floor slab is higher than that of open type composite floor. When pedestrian excitation is applied in the center of the floor slab, the peak vibration acceleration of reinforced truss concrete floor is reduced by an average of 65.65% than that of open type composite floor. After removing the bottom steel plate of the reinforced truss floor bearing slab, the fundamental frequency of the reinforced truss concrete floor is reduced by about 4.37% on average, and the peak acceleration increases by about 19.76% on average. And the thickness of the bottom steel plate also affects the vibration comfort of the floor slab to a certain extent. The increase in the thickness of the floor slab can significantly improve the vibration comfort of the reinforced truss concrete floor and the open profiled steel plate-concrete composite floor.