The development of high- speed rail cannot be separated from scientific transportation organization and management. Compared with developed countries such as Japan, Germany and France, China’s high-speed rail has a longer operating line, and the passenger flow characteristics and demands along the line vary greatly in terms of running speed, travel time, transportation price and service quality. Its transportation organization and management have unique features and world-class technical and development levels. By analyzing the characteristics and complex features of China’s high-speed rail operation management, and in combination with the main contradictions of China’s railway transportation, according to the problem-oriented and demand-driven principles, technical solutions and practices for China’s high-speed rail transportation organization are proposed. Analyzing the characteristic cases of innovation and practice in the transportation organization and management of the Yangtze River Delta high-speed rail, including the highlights of innovative breakthroughs such as optimizing transportation supply based on market demand and comprehensive solutions for the operation of busy trunk lines. Based on the operational development of high-speed railway in the Yangtze River Delta region, this paper outlines the trends in the development of China’s high-speed rail operational organization and management.